Hotspot

psbA-trnK-UUU

LSC · 1461-1888 · 428 bp

Back to Platymiscium

Pi 0.0939

MarkerSeek score 80.20

Primer available yes

Label psbA-trnK-UUU

Feature Metadata

label_name: psbA-trnK-UUU
feature_type: igs
region: LSC
length_bp: 428
variable_sites: 37
pi: 0.0939
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 80.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-trnK-UUU_p1	GCTCTCGCGTCTCTCTAAAA	ACTCCATCCGACTAGTTCCG	50.0	55.0	57.5	58.6	488-500 bp	1.0	80.7
psbA-trnK-UUU_p2	GCTCTCGCGTCTCTCTAAAA	ACTAGTTCCGGGTTCGAGTC	50.0	55.0	57.5	58.8	478-490 bp	1.0	79.1
psbA-trnK-UUU_p3	GCTCTCGCGTCTCTCTAAAA	GACTAGTTCCGGGTTCGAGT	50.0	55.0	57.5	58.8	479-491 bp	1.0	79.1
psbA-trnK-UUU_p4	GCTCTCGCGTCTCTCTAAAA	ATCCGACTAGTTCCGGGTTC	50.0	55.0	57.5	58.9	483-495 bp	1.0	78.6
psbA-trnK-UUU_p5	TCGCGTCTCTCTAAAATTGCA	ACTCCATCCGACTAGTTCCG	42.9	55.0	58.0	58.6	484-496 bp	1.0	76.8