Hotspot

clpP

LSC · 67252-69197 · 1946 bp

Back to Platylobium

Pi 0.012

MarkerSeek score 56.50

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 1946
variable_sites: 47
pi: 0.012
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 56.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	ATGCATACGGTTCACTACGT	ACGATTACGACTTCTATTAATTTCA	45.0	28.0	57.0	54.7	80-337 bp	1.0	43.0
clpP_p2	AAGAGCCGTACATGCATCTT	ACGATTACGACTTCTATTAATTTCA	45.0	28.0	57.3	54.7	80-360 bp	1.0	42.8
clpP_p3	AGAGCCGTACATGCATCTTT	ACGATTACGACTTCTATTAATTTCA	45.0	28.0	57.3	54.7	80-359 bp	1.0	42.8
clpP_p4	AAGAGCCGTACATGCATCTTT	ACGATTACGACTTCTATTAATTTCA	42.9	28.0	58.0	54.7	80-360 bp	1.0	42.8
clpP_p5	AGAGCCGTACATGCATCTTTT	ACGATTACGACTTCTATTAATTTCA	42.9	28.0	58.0	54.7	80-359 bp	1.0	42.8