Hotspot

atpB

LSC · 7089-8585 · 1497 bp

Back to Platylobium

Pi 0.00481

MarkerSeek score 58.90

Primer available yes

Label atpB

Feature Metadata

label_name: atpB
feature_type: gene
region: LSC
length_bp: 1497
variable_sites: 15
pi: 0.00481
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB_p1	CCGATCAACTAACTTTGTTAGGG	GGCAATAGGCGCATGATTTT	43.5	45.0	57.2	57.5	1698-1699 bp	1.0	46.3
atpB_p2	CCGATCAACTAACTTTGTTAGGG	TGGCAATAGGCGCATGATTT	43.5	45.0	57.2	58.6	1699-1700 bp	1.0	45.6
atpB_p3	CCGATCAACTAACTTTGTTAGGG	TGGCAATAGGCGCATGATT	43.5	47.4	57.2	57.9	1699-1700 bp	1.0	41.5
atpB_p4	ACCGATCAACTAACTTTGTTAGG	GGCAATAGGCGCATGATTTT	39.1	45.0	56.6	57.5	1699-1700 bp	1.0	41.1
atpB_p5	CCGATCAACTAACTTTGTTAGGG	GCAATAGGCGCATGATTTTGT	43.5	42.9	57.2	58.2	1697-1698 bp	1.0	40.9