Hotspot

ndhF-rpl32

SSC · 109264-109852 · 589 bp

Back to Platylobium

Pi 0.0101
MarkerSeek score 61.00
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
589
variable_sites
12
pi
0.0101
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 ACATAGGAGTTGGAAGTGGA CGTTTTGATATAGAGGTGCGC 45.0 47.6 55.5 57.5 80-688 bp 1.0 53.2
ndhF-rpl32_p2 AACATAGGAGTTGGAAGTGGA CGTTTTGATATAGAGGTGCGC 42.9 47.6 56.3 57.5 80-689 bp 1.0 51.8
ndhF-rpl32_p3 ACATAGGAGTTGGAAGTGGAA CGTTTTGATATAGAGGTGCGC 42.9 47.6 56.3 57.5 80-688 bp 1.0 51.8
ndhF-rpl32_p4 AACATAGGAGTTGGAAGTGGAA CGTTTTGATATAGAGGTGCGC 40.9 47.6 57.0 57.5 80-689 bp 1.0 49.7
ndhF-rpl32_p5 ACATAGGAGTTGGAAGTGGAAT CGTTTTGATATAGAGGTGCGC 40.9 47.6 56.8 57.5 80-688 bp 1.0 48.0