Hotspot

rps3-rps19

LSC · 80631-81203 · 573 bp

Back to Platylobium

Pi 0.00905

MarkerSeek score 58.90

Primer available yes

Label rps3-rps19

Feature Metadata

label_name: rps3-rps19
feature_type: igs
region: LSC
length_bp: 573
variable_sites: 11
pi: 0.00905
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3-rps19_p1	CAAGTCTGAAACCAAGCGGA	ACAGATCGTATGGTAGGGCA	50.0	50.0	58.4	57.9	80-716 bp	1.0	81.4
rps3-rps19_p2	AAGTCTGAAACCAAGCGGAT	ACAGATCGTATGGTAGGGCA	45.0	50.0	57.4	57.9	80-715 bp	1.0	80.1
rps3-rps19_p3	AGTCTGAAACCAAGCGGATT	ACAGATCGTATGGTAGGGCA	45.0	50.0	57.4	57.9	80-714 bp	1.0	80.1
rps3-rps19_p4	ACCAAGTCTGAAACCAAGCG	ACAGATCGTATGGTAGGGCA	50.0	50.0	58.7	57.9	80-718 bp	1.0	79.1
rps3-rps19_p5	TGGTTGTGCAAACCAAAGAG	ACAGATCGTATGGTAGGGCA	45.0	50.0	57.0	57.9	80-757 bp	1.0	76.7