Hotspot

psbI-psbK

LSC · 52476-52963 · 488 bp

Back to Platylobium

Pi 0.0217

MarkerSeek score 58.80

Primer available yes

Label psbI-psbK

Feature Metadata

label_name: psbI-psbK
feature_type: igs
region: LSC
length_bp: 488
variable_sites: 21
pi: 0.0217
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-psbK_p1	ACTACCGTGTAAACAAATAGT	TGGCAAGCTGCTGTAAGTTT	33.3	45.0	52.5	58.3	80-558 bp	1.0	46.5
psbI-psbK_p2	ACTACCGTGTAAACAAATAGT	CTTTTGTTTGGCAAGCTGCT	33.3	45.0	52.5	58.1	80-566 bp	1.0	46.4
psbI-psbK_p3	ACTACCGTGTAAACAAATAGT	GTTTGGCAAGCTGCTGTAAG	33.3	50.0	52.5	57.9	80-561 bp	1.0	46.4
psbI-psbK_p4	ACTACCGTGTAAACAAATAGTT	CTTTTGTTTGGCAAGCTGCT	31.8	45.0	53.4	58.1	80-566 bp	1.0	46.4
psbI-psbK_p5	ACTACCGTGTAAACAAATAGTT	GTTTGGCAAGCTGCTGTAAG	31.8	50.0	53.4	57.9	80-561 bp	1.0	46.4