Hotspot

trnG-GCC-trnS-GCU

LSC · 51540-52123 · 584 bp

Back to Platylobium

Pi 0.0176

MarkerSeek score 53.50

Primer available yes

Label trnG-GCC-trnS-GCU

Feature Metadata

label_name: trnG-GCC-trnS-GCU
feature_type: igs
region: LSC
length_bp: 584
variable_sites: 20
pi: 0.0176
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnS-GCU_p1	GGGTCCATAGAACGAATCACA	AGTCCACTCAGCCATCTCTC	47.6	55.0	57.5	58.5	80-650 bp	1.0	68.2
trnG-GCC-trnS-GCU_p2	AGGGTCCATAGAACGAATCAC	AGTCCACTCAGCCATCTCTC	47.6	55.0	57.2	58.5	80-651 bp	1.0	65.9
trnG-GCC-trnS-GCU_p3	GGGTCCATAGAACGAATCACA	TAGTCCACTCAGCCATCTCT	47.6	50.0	57.5	57.2	80-651 bp	1.0	65.7
trnG-GCC-trnS-GCU_p4	AAAATCAAGCAAGGGATCCC	AGTCCACTCAGCCATCTCTC	45.0	55.0	56.0	58.5	80-682 bp	1.0	63.9
trnG-GCC-trnS-GCU_p5	GGGTCCATAGAACGAATCACA	TCCACTCAGCCATCTCTCC	47.6	57.9	57.5	58.1	80-902 bp	1.0	63.3