Hotspot

trnR-UCU-trnG-GCC

LSC · 50570-50770 · 201 bp

Back to Platylobium

Pi 0.021
MarkerSeek score 59.50
Primer available yes
Label trnR-UCU-trnG-GCC

Feature Metadata

label_name
trnR-UCU-trnG-GCC
feature_type
igs
region
LSC
length_bp
201
variable_sites
8
pi
0.021
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.97
markerseek_score
59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-UCU-trnG-GCC_p1 TGCGCCCAATAGGATTTGAA AGCCTTCCAAGCTAACGATG 45.0 50.0 57.9 58.0 80-317 bp 1.0 83.1
trnR-UCU-trnG-GCC_p2 TTGCGCCCAATAGGATTTGA AGCCTTCCAAGCTAACGATG 45.0 50.0 57.9 58.0 80-318 bp 1.0 83.1
trnR-UCU-trnG-GCC_p3 TGCGCCCAATAGGATTTGAA CCTAGCCTTCCAAGCTAACG 45.0 55.0 57.9 58.1 80-320 bp 1.0 82.8
trnR-UCU-trnG-GCC_p4 TTGCGCCCAATAGGATTTGA CCTAGCCTTCCAAGCTAACG 45.0 55.0 57.9 58.1 80-321 bp 1.0 82.8
trnR-UCU-trnG-GCC_p5 TGCGCCCAATAGGATTTGAA CCCTAGCCTTCCAAGCTAAC 45.0 55.0 57.9 57.7 80-321 bp 1.0 80.6