Hotspot

trnC-GCA-rpoB

LSC · 32853-33623 · 771 bp

Back to Platylobium

Pi 0.0136

MarkerSeek score 55.50

Primer available yes

Label trnC-GCA-rpoB

Feature Metadata

label_name: trnC-GCA-rpoB
feature_type: igs
region: LSC
length_bp: 771
variable_sites: 21
pi: 0.0136
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-rpoB_p1	CACCCGGATTTGAACTAGGG	ATTCCCTCATTTTCACCCCG	55.0	50.0	58.0	57.9	80-862 bp	1.0	81.1
trnC-GCA-rpoB_p2	ACCCGGATTTGAACTAGGGA	ATTCCCTCATTTTCACCCCG	50.0	50.0	58.0	57.9	80-861 bp	1.0	81.1
trnC-GCA-rpoB_p3	CACCCGGATTTGAACTAGGG	TCAATCCGGGCAGTGTAAAC	55.0	50.0	58.0	58.2	80-882 bp	1.0	80.7
trnC-GCA-rpoB_p4	CACCCGGATTTGAACTAGGG	TCCGGGCAGTGTAAACATTC	55.0	50.0	58.0	58.2	80-878 bp	1.0	80.7
trnC-GCA-rpoB_p5	ACCCGGATTTGAACTAGGGA	TCCGGGCAGTGTAAACATTC	50.0	50.0	58.0	58.2	80-877 bp	1.0	80.7