Hotspot

trnG-UCC-psbZ

LSC · 24110-24832 · 723 bp

Back to Platylobium

Pi 0.0118

MarkerSeek score 64.10

Primer available yes

Label trnG-UCC-psbZ

Feature Metadata

label_name: trnG-UCC-psbZ
feature_type: igs
region: LSC
length_bp: 723
variable_sites: 17
pi: 0.0118
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-psbZ_p1	CGTCTTCTCCTTGGCAAAGA	CTTCTGATGGTTGGTCGAGT	50.0	50.0	57.8	57.2	350-874 bp	1.0	78.6
trnG-UCC-psbZ_p2	CGTCTTCTCCTTGGCAAAGA	TGCTTCTTCTGATGGTTGGT	50.0	45.0	57.8	57.0	350-879 bp	1.0	77.0
trnG-UCC-psbZ_p3	CGTCTTCTCCTTGGCAAAGA	TCTTCTGATGGTTGGTCGAG	50.0	50.0	57.8	57.0	350-875 bp	1.0	76.3
trnG-UCC-psbZ_p4	CGTCTTCTCCTTGGCAAAGA	TGCTTCTTCTGATGGTTGGTC	50.0	47.6	57.8	58.2	351-879 bp	1.0	74.9
trnG-UCC-psbZ_p5	CGTCTTCTCCTTGGCAAAGA	TTGCTTCTTCTGATGGTTGGT	50.0	42.9	57.8	57.8	351-880 bp	1.0	74.8