Hotspot

rps12-rrn16

IRa · 142017-148762 · 6746 bp

Back to Platycerium

Pi 0.354

MarkerSeek score 67.40

Primer available yes

Label rps12-rrn16

Feature Metadata

label_name: rps12-rrn16
feature_type: igs
region: IRa
length_bp: 6746
variable_sites: 1375
pi: 0.354
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.58
markerseek_score: 67.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-rrn16_p1	AAAGGAGAATGAGCTCGCAG	TCGTTGCCCGGACTATATCT	50.0	50.0	58.0	58.0	895 bp	0.5	59.8
rps12-rrn16_p2	AAAGGAGAATGAGCTCGCAG	TTCTAGCGCCCAGGATATGA	50.0	50.0	58.0	58.0	767 bp	0.5	59.8
rps12-rrn16_p3	AAAGGAGAATGAGCTCGCAG	CACACTGTGACCTATTCGGG	50.0	55.0	58.0	58.0	803 bp	0.5	59.7
rps12-rrn16_p4	AAAGGAGAATGAGCTCGCAG	CCACACTGTGACCTATTCGG	50.0	55.0	58.0	58.0	804 bp	0.5	59.7
rps12-rrn16_p5	GGATCTACCTGCTCCCAGAT	TCGTTGCCCGGACTATATCT	55.0	50.0	58.0	58.0	1036 bp	0.5	59.7