Hotspot

trnC-GCA-trnG-GCC

LSC · 27735-28220 · 486 bp

Back to Platycerium

Pi 0.124

MarkerSeek score 79.70

Primer available yes

Label trnC-GCA-trnG-GCC

Feature Metadata

label_name: trnC-GCA-trnG-GCC
feature_type: igs
region: LSC
length_bp: 486
variable_sites: 59
pi: 0.124
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 79.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-trnG-GCC_p1	CTCTCCTTTTAGGCGACACC	GACGTTACGGGTGGAGTTAC	55.0	55.0	58.0	58.0	868 bp	0.5	59.8
trnC-GCA-trnG-GCC_p2	CTCTCCTTTTAGGCGACACC	GGGGATTGGATGGTACGAAC	55.0	55.0	58.0	58.0	1154 bp	0.5	59.6
trnC-GCA-trnG-GCC_p3	CTCTCCTTTTAGGCGACACC	TTTCAGGGGCGTCATTATGG	55.0	50.0	58.0	57.9	976 bp	0.5	59.5
trnC-GCA-trnG-GCC_p4	AGGCTACTAAAGGGACGGTT	GACGTTACGGGTGGAGTTAC	50.0	55.0	58.1	58.0	1429 bp	0.5	59.4
trnC-GCA-trnG-GCC_p5	AGGCTACTAAAGGGACGGTT	GGGGATTGGATGGTACGAAC	50.0	55.0	58.1	58.0	1715 bp	0.5	59.3