Hotspot

psbM-petN

LSC · 26143-27120 · 978 bp

Back to Platycerium

Pi 0.093

MarkerSeek score 82.30

Primer available yes

Label psbM-petN

Feature Metadata

label_name: psbM-petN
feature_type: igs
region: LSC
length_bp: 978
variable_sites: 87
pi: 0.093
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 82.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-petN_p1	TCTTTACATTCAGACGGCCG	TTTCATGTGTCTCGATGCGT	50.0	45.0	58.0	57.9	1364-1402 bp	1.0	87.9
psbM-petN_p2	TCTTTACATTCAGACGGCCG	AAGACGGAGGACTGCAAATC	50.0	50.0	58.0	57.9	1568-1607 bp	1.0	87.2
psbM-petN_p3	TCTTTACATTCAGACGGCCG	CCTTAATGCCCCAGTTCGAA	50.0	50.0	58.0	57.9	1549-1588 bp	1.0	87.1
psbM-petN_p4	TCTTTACATTCAGACGGCCG	GGTGTCGCCTAAAAGGAGAG	50.0	55.0	58.0	58.0	1564 bp	0.5	59.9
psbM-petN_p5	GGACACGTTAGGCTGACATT	GGTGTCGCCTAAAAGGAGAG	50.0	55.0	57.9	58.0	1872 bp	0.5	59.1