Hotspot

psbI-ycf12

LSC · 6056-6777 · 722 bp

Back to Platycerium

Pi 0.0878

MarkerSeek score 79.70

Primer available yes

Label psbI-ycf12

Feature Metadata

label_name: psbI-ycf12
feature_type: igs
region: LSC
length_bp: 722
variable_sites: 62
pi: 0.0878
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 79.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-ycf12_p1	TGCCAAATTACCCGAAGCTT	CGTTACTAAGCAGTGCGACT	45.0	50.0	58.1	58.0	1856 bp	0.5	59.2
psbI-ycf12_p2	TTGCCAAATTACCCGAAGCT	CGTTACTAAGCAGTGCGACT	45.0	50.0	58.1	58.0	1857 bp	0.5	59.2
psbI-ycf12_p3	TGCCAAATTACCCGAAGCTT	TCGTTACTAAGCAGTGCGAC	45.0	50.0	58.1	58.0	1857 bp	0.5	59.1
psbI-ycf12_p4	TTGCCAAATTACCCGAAGCT	TCGTTACTAAGCAGTGCGAC	45.0	50.0	58.1	58.0	1858 bp	0.5	59.1
psbI-ycf12_p5	GTTTCGGACTGCCTCATCTT	CGTTACTAAGCAGTGCGACT	50.0	50.0	57.9	58.0	1718 bp	0.5	59.0