Hotspot

matK-chlB

LSC · 1735-3173 · 1439 bp

Back to Platycerium

Pi 0.114

MarkerSeek score 80.50

Primer available yes

Label matK-chlB

Feature Metadata

label_name: matK-chlB
feature_type: igs
region: LSC
length_bp: 1439
variable_sites: 163
pi: 0.114
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 80.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-chlB_p1	ATTTTGGTAACGGGCGCTAT	GACTTCCGAAGCTGAGTCAG	45.0	55.0	58.0	58.0	1818-1827 bp	1.0	87.9
matK-chlB_p2	CTCTAAAACGCGGCTACACT	GACTTCCGAAGCTGAGTCAG	50.0	55.0	58.0	58.0	2043 bp	0.5	59.9
matK-chlB_p3	TCTCTAAAACGCGGCTACAC	GACTTCCGAAGCTGAGTCAG	50.0	55.0	58.0	58.0	2044 bp	0.5	59.8
matK-chlB_p4	CTCTAAAACGCGGCTACACT	CGATACAACTCATGCAGCCT	50.0	50.0	58.0	58.1	2502 bp	0.5	59.5
matK-chlB_p5	CTCTAAAACGCGGCTACACT	TGTTTCTCAAGCAGCTTGGT	50.0	45.0	58.0	57.9	2577 bp	0.5	59.5