Hotspot

rpl2

IRa · 156870-158384 · 1515 bp

Back to Platycarya

Pi 0.0145

MarkerSeek score 60.40

Primer available yes

Label rpl2

Feature Metadata

label_name: rpl2
feature_type: gene
region: IRa
length_bp: 1515
variable_sites: 33
pi: 0.0145
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2_p1	CGCTTCAACCGGGTTATTCT	TGTAAAGACGAAGAAACAAATTC	50.0	30.4	58.3	53.9	1605-1627 bp	1.0	42.7
rpl2_p2	ATCATTACGCTTCAACCGGG	TGTAAAGACGAAGAAACAAATTC	50.0	30.4	58.3	53.9	1612-1634 bp	1.0	42.7
rpl2_p3	CGCTTCAACCGGGTTATTCT	TTTGTAAAGACGAAGAAACAAA	50.0	27.3	58.3	52.8	1607-1629 bp	1.0	42.7
rpl2_p4	CGCTTCAACCGGGTTATTCT	TTTTGTAAAGACGAAGAAACAA	50.0	27.3	58.3	52.8	1608-1630 bp	1.0	42.7
rpl2_p5	CGCTTCAACCGGGTTATTCT	TTTTTGTAAAGACGAAGAAACA	50.0	27.3	58.3	52.8	1609-1631 bp	1.0	42.7