Hotspot

ndhF

SSC · 113975-116200 · 2226 bp

Back to Platycarya

Pi 0.00419
MarkerSeek score 45.70
Primer available yes
Label ndhF

Feature Metadata

label_name
ndhF
feature_type
gene
region
SSC
length_bp
2226
variable_sites
14
pi
0.00419
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
45.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF_p1 TCCACTTTAAAGAGACACGCT TGGGGTTAAGTTATGGACTGTG 42.9 45.5 57.3 57.7 2979-2982 bp 0.666667 35.8
ndhF_p2 TCCACTTTAAAGAGACACGCT GGGGTTAAGTTATGGACTGTGT 42.9 45.5 57.3 57.7 2978-2981 bp 0.666667 35.8
ndhF_p3 TCCACTTTAAAGAGACACGCT CTGGGGTTAAGTTATGGACTGT 42.9 45.5 57.3 57.4 2980-2983 bp 0.666667 33.6
ndhF_p4 TTCCACTTTAAAGAGACACGCT TGGGGTTAAGTTATGGACTGTG 40.9 45.5 57.9 57.7 2980-2983 bp 0.666667 33.3
ndhF_p5 TTCCACTTTAAAGAGACACGCT GGGGTTAAGTTATGGACTGTGT 40.9 45.5 57.9 57.7 2979-2982 bp 0.666667 33.3