Hotspot

rrn5S-rrn4.5S

IRa · 135078-135302 · 225 bp

Back to Platycarya

Pi 0.0415
MarkerSeek score 68.40
Primer available yes
Label rrn5S-rrn4.5S

Feature Metadata

label_name
rrn5S-rrn4.5S
feature_type
igs
region
IRa
length_bp
225
variable_sites
14
pi
0.0415
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
68.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rrn5S-rrn4.5S_p1 AGTATCGTCACCGCAGTAGA CAGCTGAGGCATCCTAACAG 50.0 55.0 58.0 58.0 342-436 bp 1.0 82.3
rrn5S-rrn4.5S_p2 TATCGTCACCGCAGTAGAGT CAGCTGAGGCATCCTAACAG 50.0 55.0 58.0 58.0 340-434 bp 1.0 82.3
rrn5S-rrn4.5S_p3 AGTATCGTCACCGCAGTAGA GCTGAGGCATCCTAACAGAC 50.0 55.0 58.0 58.1 340-434 bp 1.0 82.3
rrn5S-rrn4.5S_p4 TATCGTCACCGCAGTAGAGT GCTGAGGCATCCTAACAGAC 50.0 55.0 58.0 58.1 338-432 bp 1.0 82.3
rrn5S-rrn4.5S_p5 TCCCCTACAGTATCGTCACC CAGCTGAGGCATCCTAACAG 55.0 55.0 57.9 58.0 350-444 bp 1.0 82.0