Hotspot

rpl32-trnL-UAG

SSC · 117311-118046 · 736 bp

Back to Platycarya

Pi 0.0261

MarkerSeek score 65.30

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 736
variable_sites: 28
pi: 0.0261
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	AGGAAGGGGTATTGGGTAGC	AGCGTGTCTACCGATTTCAC	55.0	50.0	58.5	58.0	825-867 bp	1.0	82.8
rpl32-trnL-UAG_p2	AGGAAGGGGTATTGGGTAGC	CAGCGTGTCTACCGATTTCA	55.0	50.0	58.5	58.0	826-868 bp	1.0	82.8
rpl32-trnL-UAG_p3	AGGAAGGGGTATTGGGTAGC	ATAACACTTGGCGCGGTTAT	55.0	45.0	58.5	58.0	1290-1333 bp	1.0	80.9
rpl32-trnL-UAG_p4	AGGAAGGGGTATTGGGTAGC	AAAAATAACACTTGGCGCGG	55.0	45.0	58.5	57.9	1294-1337 bp	1.0	80.7
rpl32-trnL-UAG_p5	AGGAAGGGGTATTGGGTAGC	ATGAGATTGGAGAGCGGGTA	55.0	50.0	58.5	57.9	1372-1415 bp	1.0	80.2