Hotspot

ycf1-ndhF

SSC · 113925-113974 · 50 bp

Back to Platycarya

Pi 0.293

MarkerSeek score 74.30

Primer available yes

Label ycf1-ndhF

Feature Metadata

label_name: ycf1-ndhF
feature_type: igs
region: SSC
length_bp: 50
variable_sites: 16
pi: 0.293
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-ndhF_p1	GGGAAAGCGAGGAAGAAACA	ATAGGTGGTGGCCGAATTTC	50.0	50.0	58.1	57.9	574-597 bp	1.0	84.3
ycf1-ndhF_p2	GGGAAAGCGAGGAAGAAACA	TTGTTCGTTGGAGCGATAGG	50.0	50.0	58.1	58.0	1036-1059 bp	1.0	82.7
ycf1-ndhF_p3	GGGAAAGCGAGGAAGAAACA	TCGTATCCCCATGAATCGGA	50.0	50.0	58.1	58.0	1099-1122 bp	1.0	82.5
ycf1-ndhF_p4	AGAAACAACCCCCGAAACAA	TTGTTCGTTGGAGCGATAGG	45.0	50.0	57.9	58.0	1005-1028 bp	1.0	82.5
ycf1-ndhF_p5	AGAAACAACCCCCGAAACAA	TCGTATCCCCATGAATCGGA	45.0	50.0	57.9	58.0	1068-1091 bp	1.0	82.3