Hotspot

rps12-trnV-GAC

IRb · 102144-103827 · 1684 bp

Back to Platycarya

Pi 0.0162
MarkerSeek score 72.20
Primer available yes
Label rps12-trnV-GAC

Feature Metadata

label_name
rps12-trnV-GAC
feature_type
igs
region
IRb
length_bp
1684
variable_sites
33
pi
0.0162
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
72.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps12-trnV-GAC_p1 GAGGTTAAGCGTACTCTGGC GACGGGAGAGCAAGTCAAAA 55.0 50.0 58.1 58.1 1802-1902 bp 1.0 82.6
rps12-trnV-GAC_p2 GGTTAAGCGTACTCTGGCAA GACGGGAGAGCAAGTCAAAA 50.0 50.0 57.9 58.1 1800-1900 bp 1.0 82.5
rps12-trnV-GAC_p3 GAGGTTAAGCGTACTCTGGC GATGACTTCCACCACGTCAA 55.0 50.0 58.1 57.8 1739-1839 bp 1.0 82.4
rps12-trnV-GAC_p4 GAGGTTAAGCGTACTCTGGC AACTGATGACTTCCACCACG 55.0 50.0 58.1 57.8 1743-1843 bp 1.0 82.4
rps12-trnV-GAC_p5 GAGGTTAAGCGTACTCTGGC ATGACTTCCACCACGTCAAG 55.0 50.0 58.1 57.8 1738-1838 bp 1.0 82.4