Hotspot

trnT-GGU-psbD

LSC · 34296-35634 · 1339 bp

Back to Platycarya

Pi 0.0184

MarkerSeek score 57.70

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1339
variable_sites: 36
pi: 0.0184
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	TAAGAACTCGCCAATCCGTG	55.0	50.0	58.1	58.0	1600-1848 bp	1.0	82.2
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	TAAGAACTCGCCAATCCGTG	50.0	50.0	58.1	58.0	1603-1851 bp	1.0	82.1
trnT-GGU-psbD_p3	GTGGTAGAGTAACGCCATGG	TGGACCAGAGAATGCGATTG	55.0	50.0	58.1	58.0	1847-2095 bp	1.0	81.7
trnT-GGU-psbD_p4	GTGGTAGAGTAACGCCATGG	CAAAGCTAGGCGCAAAGAAC	55.0	50.0	58.1	58.0	1920-2168 bp	1.0	81.6
trnT-GGU-psbD_p5	GTGGTAGAGTAACGCCATGG	AAAGAACCAACCCGACTGAC	55.0	50.0	58.1	58.0	1907-2155 bp	1.0	81.6