Hotspot

rpoB-trnC-GCA

LSC · 28245-29492 · 1248 bp

Back to Platycarya

Pi 0.00536

MarkerSeek score 62.90

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1248
variable_sites: 10
pi: 0.00536
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	ACTTCATTTCCACCCCCAAG	TTGTTAATCAGGCGACACCC	50.0	50.0	57.7	58.2	1344-1353 bp	1.0	77.4
rpoB-trnC-GCA_p2	ACTTCATTTCCACCCCCAAG	AAAAGGATTTGCAGTCCCCC	50.0	50.0	57.7	58.4	1308-1317 bp	1.0	76.1
rpoB-trnC-GCA_p3	ACTTCATTTCCACCCCCAAG	GCGACACCCAGATTTGAACT	50.0	50.0	57.7	58.5	1333-1342 bp	1.0	75.1
rpoB-trnC-GCA_p4	ACTTCATTTCCACCCCCAAG	TAATCAGGCGACACCCAGAT	50.0	50.0	57.7	58.5	1340-1349 bp	1.0	74.9
rpoB-trnC-GCA_p5	ACTTCATTTCCACCCCCAAG	GTTAATCAGGCGACACCCAG	50.0	55.0	57.7	58.6	1342-1351 bp	1.0	73.8