Hotspot

rpoA

LSC · 77391-78398 · 1008 bp

Back to Platostoma

Pi 0.00298

MarkerSeek score 60.50

Primer available yes

Label rpoA

Feature Metadata

label_name: rpoA
feature_type: gene
region: LSC
length_bp: 1008
variable_sites: 3
pi: 0.00298
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA_p1	TGAATTTGCTACAGATTCAAATCT	AATGGCTGTAGACCTCCGAA	29.2	50.0	55.1	58.4	1172 bp	1.0	40.2
rpoA_p2	TGAATTTGCTACAGATTCAAATCT	ATGGCTGTAGACCTCCGAAA	29.2	50.0	55.1	58.4	1171 bp	1.0	40.2
rpoA_p3	TGAATTTGCTACAGATTCAAATCT	TAATGGCTGTAGACCTCCGA	29.2	50.0	55.1	57.6	1173 bp	1.0	40.2
rpoA_p4	TGAATTTGCTACAGATTCAAATCT	GGCTGTAGACCTCCGAAAAA	29.2	50.0	55.1	57.5	1169 bp	1.0	40.2
rpoA_p5	TGAATTTGCTACAGATTCAAATCT	TGGCTGTAGACCTCCGAAAA	29.2	50.0	55.1	58.7	1170 bp	1.0	40.2