Hotspot

rps15-ycf1

SSC · 121945-122390 · 446 bp

Back to Platostoma

Pi 0.0045

MarkerSeek score 58.60

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 446
variable_sites: 2
pi: 0.0045
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	ACTTGAAATTCAACAGACCCTCT	ACCCATAATGGCAGTCGTTT	39.1	45.0	57.9	57.5	590-592 bp	1.0	52.1
rps15-ycf1_p2	ACTTGAAATTCAACAGACCCTC	ACCCATAATGGCAGTCGTTT	40.9	45.0	56.5	57.5	590-592 bp	1.0	48.8
rps15-ycf1_p3	ACTTGAAATTCAACAGACCCTCT	CCCATAATGGCAGTCGTTTC	39.1	50.0	57.9	56.9	589-591 bp	1.0	47.0
rps15-ycf1_p4	ACTTGAAATTCAACAGACCCT	ACCCATAATGGCAGTCGTTT	38.1	45.0	55.3	57.5	590-592 bp	1.0	47.0
rps15-ycf1_p5	ACTTGAAATTCAACAGACCCTCT	AGACTACATCTGTATCCGCGA	39.1	47.6	57.9	58.2	560-562 bp	1.0	46.9