Hotspot

rpl2-rpl23

IRb · 85075-85092 · 18 bp

Back to Platostoma

Pi 0.0556

MarkerSeek score 81.90

Primer available yes

Label rpl2-rpl23

Feature Metadata

label_name: rpl2-rpl23
feature_type: igs
region: IRb
length_bp: 18
variable_sites: 1
pi: 0.0556
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2-rpl23_p1	GTTTACGGTTCCATTGCGTG	CGCTTCAACCGGGTTATTCT	50.0	50.0	58.0	58.3	125 bp	1.0	78.2
rpl2-rpl23_p2	GTTTACGGTTCCATTGCGTG	ATCATTACGCTTCAACCGGG	50.0	50.0	58.0	58.3	132 bp	1.0	77.6
rpl2-rpl23_p3	GTTTACGGTTCCATTGCGTG	TCCGGGAAAGGGTAGAAGAA	50.0	50.0	58.0	57.7	193 bp	1.0	77.4
rpl2-rpl23_p4	GTTTACGGTTCCATTGCGTG	TTCCGGGAAAGGGTAGAAGA	50.0	50.0	58.0	57.7	194 bp	1.0	77.4
rpl2-rpl23_p5	TGCTCGGGGTAGAAGTTTTG	CGCTTCAACCGGGTTATTCT	50.0	50.0	57.8	58.3	105 bp	1.0	77.2