Hotspot

petA-psbJ

LSC · 62986-64008 · 1023 bp

Back to Platostoma

Pi 0.00198

MarkerSeek score 61.00

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1023
variable_sites: 2
pi: 0.00198
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GATCCACTACGTGTCCAAGG	TCCGGATTAGGTTCATCCCT	55.0	50.0	57.7	57.5	1155-1167 bp	1.0	74.7
petA-psbJ_p2	GATCCACTACGTGTCCAAGG	CCGGATTAGGTTCATCCCTG	55.0	55.0	57.7	57.4	1154-1166 bp	1.0	74.2
petA-psbJ_p3	ATCCACTACGTGTCCAAGGT	TCCGGATTAGGTTCATCCCT	50.0	50.0	58.4	57.5	1154-1166 bp	1.0	74.2
petA-psbJ_p4	ATCCACTACGTGTCCAAGGT	CCGGATTAGGTTCATCCCTG	50.0	55.0	58.4	57.4	1153-1165 bp	1.0	73.7
petA-psbJ_p5	CACTACGTGTCCAAGGTCTT	TCCGGATTAGGTTCATCCCT	50.0	50.0	57.2	57.5	1151-1163 bp	1.0	70.5