Hotspot

rbcL-accD

LSC · 56447-57089 · 643 bp

Back to Platostoma

Pi 0.00156

MarkerSeek score 59.60

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 643
variable_sites: 1
pi: 0.00156
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	CCGCTTGTGAGGTATGGAAA	ACCCGCATTCTAACTCCTTC	50.0	50.0	57.9	57.3	823 bp	1.0	73.7
rbcL-accD_p2	CCGTGAAGCTACCAAATGGA	ACCCGCATTCTAACTCCTTC	50.0	50.0	57.9	57.3	858 bp	1.0	73.7
rbcL-accD_p3	TCCGTGAAGCTACCAAATGG	ACCCGCATTCTAACTCCTTC	50.0	50.0	57.9	57.3	859 bp	1.0	73.7
rbcL-accD_p4	CCGCTTGTGAGGTATGGAAA	CCCATACCCGCATTCTAACT	50.0	50.0	57.9	57.1	828 bp	1.0	71.8
rbcL-accD_p5	CCGTGAAGCTACCAAATGGA	CCCATACCCGCATTCTAACT	50.0	50.0	57.9	57.1	863 bp	1.0	71.8