Hotspot

rps4-trnT-UGU

LSC · 45862-46261 · 400 bp

Back to Platostoma

Pi 0.0026

MarkerSeek score 59.90

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 400
variable_sites: 1
pi: 0.0026
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	TCTAAGATCACTCCCGGCTT	CTCAGAGGTTAGAGCATCGC	50.0	55.0	57.8	57.9	546-562 bp	1.0	80.1
rps4-trnT-UGU_p2	TTCTAAGATCACTCCCGGCT	CTCAGAGGTTAGAGCATCGC	50.0	55.0	57.8	57.9	547-563 bp	1.0	80.1
rps4-trnT-UGU_p3	TCTAAGATCACTCCCGGCTT	ATGGTCATCGGTTCGATTCC	50.0	50.0	57.8	57.8	514-530 bp	1.0	79.4
rps4-trnT-UGU_p4	TTCTAAGATCACTCCCGGCT	ATGGTCATCGGTTCGATTCC	50.0	50.0	57.8	57.8	515-531 bp	1.0	79.4
rps4-trnT-UGU_p5	TCTAAGATCACTCCCGGCTT	TACTATAATGTGGGCCCGCT	50.0	50.0	57.8	58.3	569-585 bp	1.0	78.9