Hotspot

psaA-ycf3

LSC · 41293-42042 · 750 bp

Back to Platostoma

Pi 0

MarkerSeek score 60.00

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 750
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GAAAAATGACCCGGTTTGGC	ATTGGTTGAAGATCACGGGG	50.0	50.0	58.2	57.9	870-880 bp	1.0	78.2
psaA-ycf3_p2	GAAAAATGACCCGGTTTGGC	TGGTTTGATCAAGCTGCTGA	50.0	45.0	58.2	57.7	943-953 bp	1.0	77.0
psaA-ycf3_p3	TTTTACTTCTGGTTCCGGCG	ATTGGTTGAAGATCACGGGG	50.0	50.0	58.5	57.9	805-815 bp	1.0	76.1
psaA-ycf3_p4	GAAAAATGACCCGGTTTGGC	CGGAGGCTTGGTTTGATCAA	50.0	50.0	58.2	58.5	951-961 bp	1.0	75.4
psaA-ycf3_p5	TTGGCCCATTCTTCGAAAGA	ATTGGTTGAAGATCACGGGG	45.0	50.0	57.4	57.9	855-865 bp	1.0	75.3