Hotspot

ndhD

SSC · 122833-124335 · 1503 bp

Back to Platanus

Pi 0.00133

MarkerSeek score 44.50

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1503
variable_sites: 3
pi: 0.00133
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	ACGAACGATTTTTCAAATTACAGGA	TTTCGAGCACGGGTTTTTCT	32.0	45.0	58.0	58.3	1591-1592 bp	1.0	40.2
ndhD_p2	ACGAACGATTTTTCAAATTACAGG	TTTCGAGCACGGGTTTTTCT	33.3	45.0	56.8	58.3	1591-1592 bp	1.0	40.2
ndhD_p3	ACGAACGATTTTTCAAATTACAGGA	ATTTCGAGCACGGGTTTTTC	32.0	45.0	58.0	57.3	1592-1593 bp	1.0	40.2
ndhD_p4	ACAACGAACGATTTTTCAAA	TTTCGAGCACGGGTTTTTCT	30.0	45.0	52.6	58.3	1594-1595 bp	1.0	40.2
ndhD_p5	CGAACGATTTTTCAAATTACAGGA	TTTCGAGCACGGGTTTTTCT	33.3	45.0	56.5	58.3	1590-1591 bp	1.0	40.2