Hotspot

atpF

LSC · 13344-14665 · 1322 bp

Back to Platanus

Pi 0.00202

MarkerSeek score 43.60

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1322
variable_sites: 4
pi: 0.00202
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	GCTCGAAGGGTTACCATGAG	AGGGCGAAGTGATACAAAAAGA	55.0	40.9	58.1	58.0	1463-1468 bp	1.0	63.9
atpF_p2	GCTCGAAGGGTTACCATGAG	GGGCGAAGTGATACAAAAAGA	55.0	42.9	58.1	56.5	1462-1467 bp	1.0	60.3
atpF_p3	GCTCGAAGGGTTACCATGAG	AGGGCGAAGTGATACAAAAAG	55.0	42.9	58.1	56.5	1463-1468 bp	1.0	60.2
atpF_p4	ACTAATTTCGTCGGCTCGAA	AGGGCGAAGTGATACAAAAAGA	45.0	40.9	57.4	58.0	1476-1481 bp	1.0	59.2
atpF_p5	CGAAGGGTTACCATGAGTGT	AGGGCGAAGTGATACAAAAAGA	50.0	40.9	57.2	58.0	1460-1465 bp	1.0	58.3