Hotspot

trnL-UAG-ccsA

SSC · 121514-121618 · 105 bp

Back to Platanus

Pi 0.0129
MarkerSeek score 49.50
Primer available yes
Label trnL-UAG-ccsA

Feature Metadata

label_name
trnL-UAG-ccsA
feature_type
igs
region
SSC
length_bp
105
variable_sites
2
pi
0.0129
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
49.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAG-ccsA_p1 GTGAAATCGGTAGACACGCT CCATCGACTAATAGGGTCATCA 50.0 45.5 58.0 56.9 272-274 bp 1.0 56.0
trnL-UAG-ccsA_p2 TGAAATCGGTAGACACGCTG CCATCGACTAATAGGGTCATCA 50.0 45.5 58.0 56.9 271-273 bp 1.0 56.0
trnL-UAG-ccsA_p3 GTGAAATCGGTAGACACGCT TCCATCGACTAATAGGGTCA 50.0 45.0 58.0 54.7 273-275 bp 1.0 55.1
trnL-UAG-ccsA_p4 TGAAATCGGTAGACACGCTG TCCATCGACTAATAGGGTCA 50.0 45.0 58.0 54.7 272-274 bp 1.0 55.1
trnL-UAG-ccsA_p5 TAGACACGCTGCTCTTAGGA CCATCGACTAATAGGGTCATCA 50.0 45.5 57.9 56.9 262-264 bp 1.0 55.1