Hotspot

ycf1-ndhF

SSC · 117217-117294 · 78 bp

Back to Platanus

Pi 0.094

MarkerSeek score 75.00

Primer available yes

Label ycf1-ndhF

Feature Metadata

label_name: ycf1-ndhF
feature_type: igs
region: SSC
length_bp: 78
variable_sites: 11
pi: 0.094
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-ndhF_p1	TCTAGCATTGGGTAGACCTCA	GCGGGCGAATCTCTTCTTAT	47.6	50.0	57.6	57.8	270 bp	1.0	70.8
ycf1-ndhF_p2	GCATTGGGTAGACCTCATACA	GCGGGCGAATCTCTTCTTAT	47.6	50.0	57.2	57.8	266 bp	1.0	67.4
ycf1-ndhF_p3	CTAGCATTGGGTAGACCTCA	GCGGGCGAATCTCTTCTTAT	50.0	50.0	56.0	57.8	269 bp	1.0	66.3
ycf1-ndhF_p4	TCTAGCATTGGGTAGACCTCAT	GCGGGCGAATCTCTTCTTAT	45.5	50.0	58.1	57.8	270 bp	1.0	65.4
ycf1-ndhF_p5	TCTAGCATTGGGTAGACCTCA	ATCAAATATGTAGGGGGCGG	47.6	50.0	57.6	57.1	286 bp	1.0	64.9