Hotspot

accD-psaI

LSC · 65822-66453 · 632 bp

Back to Platanus

Pi 0.00106

MarkerSeek score 47.40

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 632
variable_sites: 1
pi: 0.00106
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	CCACGGTTTCTTTCCCTTGA	AAAGAGGCCATTGCAATTGC	50.0	45.0	58.0	57.9	749-752 bp	1.0	79.4
accD-psaI_p2	TCCACGGTTTCTTTCCCTTG	AAAGAGGCCATTGCAATTGC	50.0	45.0	58.0	57.9	750-753 bp	1.0	79.4
accD-psaI_p3	TCAGCTCCACGGTTTCTTTC	AAAGAGGCCATTGCAATTGC	50.0	45.0	58.1	57.9	755-758 bp	1.0	78.6
accD-psaI_p4	CCACGGTTTCTTTCCCTTGA	TGCAATTGCCGGCAATACTA	50.0	45.0	58.0	58.2	738-741 bp	1.0	78.3
accD-psaI_p5	TCCACGGTTTCTTTCCCTTG	TGCAATTGCCGGCAATACTA	50.0	45.0	58.0	58.2	739-742 bp	1.0	78.3