Hotspot

ndhC-trnV-UAC

LSC · 55978-58243 · 2266 bp

Back to Platanus

Pi 0.00266
MarkerSeek score 44.70
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
2266
variable_sites
9
pi
0.00266
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
44.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 CTCTGGTCCTTCGCTAATCG AGAAGGTCTACGGTTCGAGT 55.0 50.0 57.9 57.8 2416-2432 bp 1.0 78.0
ndhC-trnV-UAC_p2 CTCTGGTCCTTCGCTAATCG GAAGGTCTACGGTTCGAGTC 55.0 55.0 57.9 57.5 2415-2431 bp 1.0 75.3
ndhC-trnV-UAC_p3 CTAATCGGGGCCAAAACTCC AGAAGGTCTACGGTTCGAGT 55.0 50.0 58.6 57.8 2403-2419 bp 1.0 74.2
ndhC-trnV-UAC_p4 CTCTGGTCCTTCGCTAATCG GTTCGAGTCCGTATAGCCCT 55.0 55.0 57.9 58.7 2404-2420 bp 1.0 74.1
ndhC-trnV-UAC_p5 CTCTGGTCCTTCGCTAATCG AAGGTCTACGGTTCGAGTCC 55.0 55.0 57.9 58.8 2414-2430 bp 1.0 73.0