Pi
0.00611
MarkerSeek score
47.80
Primer available
yes
Label
trnT-UGU-trnL-UAA
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| trnT-UGU-trnL-UAA_p1 | AAAGCCAGCTATCGGAATCG | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 58.1 | 58.0 | 1514-1517 bp | 1.0 | 79.7 |
| trnT-UGU-trnL-UAA_p2 | CTCTAACCGCTGAGCTAAGC | ACCAATTTCGCCATATCCCC | 55.0 | 50.0 | 58.2 | 58.0 | 1463-1466 bp | 1.0 | 79.5 |
| trnT-UGU-trnL-UAA_p3 | AAAGCCAGCTATCGGAATCG | TAGCGTCTACCAATTTCGCC | 50.0 | 50.0 | 58.1 | 58.1 | 1522-1525 bp | 1.0 | 79.1 |
| trnT-UGU-trnL-UAA_p4 | CTCTAACCGCTGAGCTAAGC | TAGCGTCTACCAATTTCGCC | 55.0 | 50.0 | 58.2 | 58.1 | 1471-1474 bp | 1.0 | 79.0 |
| trnT-UGU-trnL-UAA_p5 | GGAATCGAACCGATGACCAT | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 57.8 | 58.0 | 1501-1504 bp | 1.0 | 78.8 |