Hotspot

rpoA

LSC · 77093-78079 · 987 bp

Back to Platanthera

Pi 0.0101

MarkerSeek score 45.30

Primer available yes

Label rpoA

Feature Metadata

label_name: rpoA
feature_type: gene
region: LSC
length_bp: 987
variable_sites: 22
pi: 0.0101
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA_p1	CGTCGTCCAGTAGCTACAAC	GCAACGAGCAGAGGTTATGA	55.0	50.0	58.1	58.0	1545-1561 bp	1.0	82.4
rpoA_p2	CGTCGTCCAGTAGCTACAAC	TCACGTTCAAGCGAGTTTCA	55.0	45.0	58.1	58.1	1719-1735 bp	1.0	81.7
rpoA_p3	GTTCCTTTGGGGGATGGATC	GCAACGAGCAGAGGTTATGA	55.0	50.0	57.9	58.0	1955-1968 bp	0.833333	75.1
rpoA_p4	GTTCCTTTGGGGGATGGATC	TCACGTTCAAGCGAGTTTCA	55.0	45.0	57.9	58.1	2129-2142 bp	0.833333	74.5
rpoA_p5	GTTCCTTTGGGGGATGGATC	GAACACCCTATGCTGCTCAA	55.0	50.0	57.9	57.9	2019-2032 bp	0.833333	74.2