Hotspot

rps3-rpl22

LSC · 82845-82930 · 86 bp

Back to Platanthera

Pi 0.0512

MarkerSeek score 71.50

Primer available yes

Label rps3-rpl22

Feature Metadata

label_name: rps3-rpl22
feature_type: igs
region: LSC
length_bp: 86
variable_sites: 9
pi: 0.0512
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3-rpl22_p1	TGAGTCTCCGGTGAAACTCT	CTTTCGTGAGACATGCGAGA	50.0	50.0	58.0	58.0	999-1012 bp	1.0	84.0
rps3-rpl22_p2	TGAGTCTCCGGTGAAACTCT	CAGATCGTATGGTCGGTCAC	50.0	55.0	58.0	57.9	1047-1060 bp	1.0	83.0
rps3-rpl22_p3	TGATCCATTCAACACGTGCA	CTTTCGTGAGACATGCGAGA	45.0	50.0	58.1	58.0	1206-1219 bp	1.0	82.8
rps3-rpl22_p4	ACGTGCAATCTCTTTTCCGT	CTTTCGTGAGACATGCGAGA	45.0	50.0	58.1	58.0	1193-1206 bp	1.0	82.8
rps3-rpl22_p5	CCGCGATTTGCACTTGAATT	CTTTCGTGAGACATGCGAGA	45.0	50.0	58.0	58.0	1174-1177 bp	0.5	60.0