Hotspot

petD-rpoA

LSC · 76866-77092 · 227 bp

Back to Platanthera

Pi 0.0536

MarkerSeek score 74.30

Primer available yes

Label petD-rpoA

Feature Metadata

label_name: petD-rpoA
feature_type: igs
region: LSC
length_bp: 227
variable_sites: 27
pi: 0.0536
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 74.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-rpoA_p1	CGTCGTCCAGTAGCTACAAC	TTGGGCACTTTACAGAAGCA	55.0	45.0	58.1	57.6	354-370 bp	1.0	84.9
petD-rpoA_p2	TCGTCGTCCAGTAGCTACAA	TTGGGCACTTTACAGAAGCA	50.0	45.0	58.2	57.6	355-371 bp	1.0	84.2
petD-rpoA_p3	TTCGTCGTCCAGTAGCTACA	TTGGGCACTTTACAGAAGCA	50.0	45.0	58.2	57.6	356-372 bp	1.0	84.2
petD-rpoA_p4	AATTGGTACCGCAGTAGCTC	TTGGGCACTTTACAGAAGCA	50.0	45.0	57.7	57.6	325-341 bp	1.0	84.0
petD-rpoA_p5	CATTTCGTCGTCCAGTAGCT	TTGGGCACTTTACAGAAGCA	50.0	45.0	57.7	57.6	359-375 bp	1.0	83.4