Pi
0.0265
MarkerSeek score
76.60
Primer available
yes
Label
trnT-UGU-trnL-UAA
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| trnT-UGU-trnL-UAA_p1 | AGAAAAAGAGCCGGCTATCG | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 58.1 | 58.0 | 1019-1061 bp | 1.0 | 88.0 |
| trnT-UGU-trnL-UAA_p2 | GCGATGCTCTAACCTCTGAG | ACCAATTTCGCCATATCCCC | 55.0 | 50.0 | 57.9 | 58.0 | 968-1010 bp | 1.0 | 87.7 |
| trnT-UGU-trnL-UAA_p3 | AGAAAAAGAGCCGGCTATCG | TAGCGTCTACCAATTTCGCC | 50.0 | 50.0 | 58.1 | 58.1 | 1027-1069 bp | 1.0 | 87.5 |
| trnT-UGU-trnL-UAA_p4 | GCGATGCTCTAACCTCTGAG | TAGCGTCTACCAATTTCGCC | 55.0 | 50.0 | 57.9 | 58.1 | 976-1018 bp | 1.0 | 87.1 |
| trnT-UGU-trnL-UAA_p5 | GGAATCGAACCGATGACCAT | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 57.8 | 58.0 | 1000-1042 bp | 1.0 | 86.7 |