Hotspot

trnT-GGU-psbD

LSC · 30962-31979 · 1018 bp

Back to Platanthera

Pi 0.0238

MarkerSeek score 61.40

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1018
variable_sites: 52
pi: 0.0238
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	GACCAGCCTACAAACACGAA	55.0	50.0	58.1	58.1	1160-1177 bp	1.0	84.8
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	GACCAGCCTACAAACACGAA	50.0	50.0	58.1	58.1	1163-1180 bp	1.0	84.6
trnT-GGU-psbD_p3	GTGGTAGAGTAACGCCATGG	CCCTCCGTAACCAATCATCC	55.0	55.0	58.1	57.7	1135-1152 bp	1.0	83.9
trnT-GGU-psbD_p4	TCAGTGGTAGAGTAACGCCA	CCCTCCGTAACCAATCATCC	50.0	55.0	58.1	57.7	1138-1155 bp	1.0	83.7
trnT-GGU-psbD_p5	GTGGTAGAGTAACGCCATGG	CAGCCTACAAACACGAAACG	55.0	50.0	58.1	57.7	1157-1174 bp	1.0	83.5