Hotspot

trnK-UUU-rps16

LSC · 4252-4898 · 647 bp

Back to Platanthera

Pi 0.0304
MarkerSeek score 64.00
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
647
variable_sites
38
pi
0.0304
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT TCGATCCCGAAGAGAAGGAA 50.0 50.0 58.2 57.9 830-863 bp 1.0 84.2
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT TCGATCCCGAAGAGAAGGAA 50.0 50.0 58.2 57.9 829-862 bp 1.0 84.2
trnK-UUU-rps16_p3 CAGGATCAGTCGCGGTATTC TCGATCCCGAAGAGAAGGAA 55.0 50.0 58.2 57.9 916-949 bp 1.0 83.2
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT CCTTGAAAAAGGCGCTCAAC 50.0 50.0 58.2 58.2 733-755 bp 0.666667 68.5
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT CTTGAAAAAGGCGCTCAACC 50.0 50.0 58.2 58.2 732-754 bp 0.666667 68.5