Hotspot

rrn16

IRb · 97790-99280 · 1491 bp

Back to Plantago

Pi 0.203

MarkerSeek score 66.30

Primer available yes

Label rrn16

Feature Metadata

label_name: rrn16
feature_type: rRNA
region: IRb
length_bp: 1491
variable_sites: 714
pi: 0.203
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 66.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn16_p1	GAAGACCCGGCTCTAAGTTG	GGACGTAACAAAGGGGATCC	55.0	55.0	58.0	58.0	2071-2087 bp	1.0	91.6
rrn16_p2	GAAGACCCGGCTCTAAGTTG	GGGACGTAACAAAGGGGATC	55.0	55.0	58.0	58.0	2072-2088 bp	1.0	91.6
rrn16_p3	GAAGACCCGGCTCTAAGTTG	GGCAAACCAAAAGACCCAAC	55.0	50.0	58.0	58.1	1915-1931 bp	1.0	91.5
rrn16_p4	GAAGACCCGGCTCTAAGTTG	AGACGTAGCTCCCCTCTTTT	55.0	50.0	58.0	58.1	1955-1971 bp	1.0	91.4
rrn16_p5	GAAGACCCGGCTCTAAGTTG	ACGTTTCACTCAGACGTAGC	55.0	50.0	58.0	58.0	1966-1982 bp	0.404255	67.7