Hotspot

rps7

IRa · 137068-137535 · 468 bp

Back to Plantago

Pi 0.22

MarkerSeek score 61.90

Primer available yes

Label rps7

Feature Metadata

label_name: rps7
feature_type: gene
region: IRa
length_bp: 468
variable_sites: 245
pi: 0.22
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps7_p1	CCCTAGATGCTGTCGGAGTA	CTCTCCATCGGAACAACAGG	55.0	55.0	58.0	58.0	1636-1648 bp	0.893617	87.5
rps7_p2	AGTTTCGTAAGGGGACTGGA	CTCTCCATCGGAACAACAGG	50.0	55.0	58.0	58.0	1496-1508 bp	0.893617	87.5
rps7_p3	GCGTTCTAGTGCGTTGTAGA	CTCTCCATCGGAACAACAGG	50.0	55.0	58.0	58.0	1599-1611 bp	0.893617	87.4
rps7_p4	TTTCCCTGACTTTGTCCGTG	CTCTCCATCGGAACAACAGG	50.0	55.0	58.0	58.0	1381-1393 bp	0.893617	87.3
rps7_p5	CCTGACTTTGTCCGTGTCAA	CTCTCCATCGGAACAACAGG	50.0	55.0	58.1	58.0	1377-1389 bp	0.893617	87.2