Hotspot

ndhF

SSC · 107115-109346 · 2232 bp

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Pi 0.0547

MarkerSeek score 76.80

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2232
variable_sites: 496
pi: 0.0547
unique_haplotype_count: 45
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	AATACCGTTCCCCATGCAAA	CGGCGCTTTGCTAATTCATT	45.0	45.0	57.8	57.8	2923-2995 bp	0.06383	46.7
ndhF_p2	TACCGTTCCCCATGCAAATC	CGGCGCTTTGCTAATTCATT	50.0	45.0	58.2	57.8	2921-2993 bp	0.06383	46.5
ndhF_p3	GCCACAGCTGATGAAGATGA	CGGCGCTTTGCTAATTCATT	50.0	45.0	58.0	57.8	2877-2981 bp	0.06383	42.1
ndhF_p4	TTTTCCTGTGCCTACGCTTT	CGGCGCTTTGCTAATTCATT	45.0	45.0	58.0	57.8	2949-2950 bp	0.042553	40.0
ndhF_p5	TTTTTCCTGTGCCTACGCTT	CGGCGCTTTGCTAATTCATT	45.0	45.0	58.0	57.8	2950-2951 bp	0.042553	40.0