Hotspot

ndhB

IRa · 137865-140026 · 2162 bp

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Pi 0.206

MarkerSeek score 66.40

Primer available yes

Label ndhB

Feature Metadata

label_name: ndhB
feature_type: gene
region: IRa
length_bp: 2162
variable_sites: 1066
pi: 0.206
unique_haplotype_count: 22
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhB_p1	ATGGCAGAGGCAAATAGAGC	GGACGACGTAGGGATCCATA	50.0	55.0	58.0	58.1	2764-2773 bp	0.361702	65.5
ndhB_p2	TTGGTTATTAGCGGCATCCC	GGACGACGTAGGGATCCATA	50.0	55.0	58.0	58.1	2888-2897 bp	0.361702	65.5
ndhB_p3	GGCAGAGGCAAATAGAGCTT	GGACGACGTAGGGATCCATA	50.0	55.0	58.0	58.1	2762-2771 bp	0.361702	65.3
ndhB_p4	ATGGCAGAGGCAAATAGAGC	TCGGTCATATAAGCCCGAGT	50.0	50.0	58.0	58.0	2944-2992 bp	0.191489	49.3
ndhB_p5	GGCAGAGGCAAATAGAGCTT	TCGGTCATATAAGCCCGAGT	50.0	50.0	58.0	58.0	2942-2990 bp	0.191489	49.1