Hotspot

clpP

LSC · 68355-69723 · 1369 bp

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Pi 0.103

MarkerSeek score 75.20

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 1369
variable_sites: 501
pi: 0.103
unique_haplotype_count: 39
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 75.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	TAGCTGACCCTCATAGTCCG	GAGTAAATGCGTCTGTCCGT	55.0	50.0	58.0	58.0	2088-2772 bp	0.234043	61.2
clpP_p2	ATCCCCAACCGCATATTTCC	GAGTAAATGCGTCTGTCCGT	50.0	50.0	58.0	58.0	1559-1582 bp	0.106383	51.9
clpP_p3	ATCCCCAACCGCATATTTCC	TGAGTAAATGCGTCTGTCCG	50.0	50.0	58.0	58.0	1583 bp	0.042553	41.6
clpP_p4	TAGCTGACCCTCATAGTCCG	TGAGTAAATGCGTCTGTCCG	55.0	50.0	58.0	58.0	2113 bp	0.042553	41.5
clpP_p5	ATCCCCAACCGCATATTTCC	CTGTAATACTCCAACCGCCC	50.0	55.0	58.0	58.1	2049 bp	0.042553	41.3