Hotspot

rpl32-trnL-UAG

SSC · 110199-110804 · 606 bp

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Pi 0.0966

MarkerSeek score 79.70

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 606
variable_sites: 281
pi: 0.0966
unique_haplotype_count: 44
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 79.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	AAAAGGAAGGGGTATTGGGC	GCCCCTTTCGGATGAATCAT	50.0	50.0	57.8	58.0	939-1238 bp	0.914894	86.5
rpl32-trnL-UAG_p2	AAAAGCGTTTTCCTTGGGGA	ATGCCCCTTTCGGATGAATC	45.0	50.0	58.2	58.0	993-1214 bp	0.382979	65.5
rpl32-trnL-UAG_p3	AAAAGCGTTTTCCTTGGGGA	GCCCCTTTCGGATGAATCAT	45.0	50.0	58.2	58.0	991-1212 bp	0.340426	63.8
rpl32-trnL-UAG_p4	AATGAATTAGCAAAGCGCCG	GCCCCTTTCGGATGAATCAT	45.0	50.0	57.8	58.0	1168-1232 bp	0.12766	54.3
rpl32-trnL-UAG_p5	AATGAATTAGCAAAGCGCCG	ATGCCCCTTTCGGATGAATC	45.0	50.0	57.8	58.0	1170-1234 bp	0.12766	54.2