Hotspot

ndhF-rpl32

SSC · 109347-110033 · 687 bp

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Pi 0.083

MarkerSeek score 79.40

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 687
variable_sites: 273
pi: 0.083
unique_haplotype_count: 43
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 79.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TCGTTGCTGTCGGAAAAAGA	TTGATTCCAACCCTTCAGGC	45.0	50.0	58.1	58.1	975-976 bp	0.042553	40.7
ndhF-rpl32_p2	GCCCACATACGACGAAGATT	TTGATTCCAACCCTTCAGGC	50.0	50.0	58.1	58.1	997-998 bp	0.042553	40.7
ndhF-rpl32_p3	ACTCGGAAAAGCCCACATAC	TTGATTCCAACCCTTCAGGC	50.0	50.0	57.9	58.1	1007-1008 bp	0.042553	40.3
ndhF-rpl32_p4	TCTCAAGTAGCCCTGATCGT	TTGATTCCAACCCTTCAGGC	50.0	50.0	57.9	58.1	1244-1245 bp	0.042553	40.0
ndhF-rpl32_p5	TCAAGTAGCCCTGATCGTGA	TTGATTCCAACCCTTCAGGC	50.0	50.0	58.2	58.1	1242-1243 bp	0.042553	39.9